Methods of in vitro protein synthesis

US9410170B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Kara Calhoun · South San Francisco, US
• Michael Christopher Jewett · Evanston, US
• James R. Swartz · Menlo Park, US

Key dates

• Filing date: Nov 18, 2004
• Grant date: Aug 9, 2016
• Expiry date: Feb 5, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Improved methods are provided in vitro synthesis of biological molecules, providing for improved yields, lowered costs, and enhanced utility. Improved yield and lowered cost is obtained by the use of a phosphate free energy source in the presence of exogenous phosphate, and optionally in the absence of exogenous nucleoside triphosphates.