Template switch-based methods for producing a product nucleic acid
US9410173B2 · kind B2 · utility
60Cited by
4References
15Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 18, 2013 |
| Grant date | Aug 9, 2016 |
| Priority date | — |
| Expiry date | Oct 18, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2525/191
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided are methods of producing a product nucleic acid. The methods include combining a template deoxyribonucleic acid (DNA), a polymerase, a template switch oligonucleotide, and dNTPs into a reaction mixture. The components are combined into the reaction mixture under conditions sufficient to produce a product nucleic acid that includes the template DNA and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid that includes a region polymerized from the dNTPs by the polymerase. Aspects of the invention further include compositions and kits.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.