Method for producing proteins

US9410176B2 · kind B2 · utility

Assignees

• Inter-University Research Institute Corporation Research Organization of Information and Systems · JP
• KYOWA HAKKO KIRIN CO., LTD. · JP

Inventors

• Megumi Kurokawa · Tokyo, JP
• Yoko Hayashi · Kawasaki, JP
• Masayoshi TSUKAHARA · Shibukawa, JP

Key dates

• Filing date: Dec 14, 2011
• Grant date: Aug 9, 2016
• Expiry date: Dec 14, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2800/90
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention relates to a method for producing a protein of interest, comprising introducing an expression vector which comprises a gene fragment comprising a DNA encoding the protein of interest and a selectable marker gene and also comprises a pair of transposon sequences at both terminals of the gene fragment, into a suspension mammalian cell; integrating the gene fragment inserted between the pair of transposon sequences into a chromosome of the mammalian cell; obtaining a suspension mammalian cell producing the protein of interest; and suspension-culturing the suspension mammalian cell, and a suspension mammalian cell which expresses the protein of interest by the method.