Alternative nucleotide flows in sequencing-by-synthesis methods
US9416413B2 · kind B2 · utility
6Cited by
36References
11Claims
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Key dates
| Filing date | Apr 9, 2013 |
| Grant date | Aug 16, 2016 |
| Priority date | — |
| Expiry date | Apr 9, 2033 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L3/5027
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for sequencing a polynucleotide strand by using sequencing-by-synthesis techniques. To address the problem of incomplete extension (IE) and/or carry forward (CF) errors that can occur in sequencing-by-synthesis reactions, an alternative flow ordering of dNTPs is used. In contrast to conventional flow orderings, the dNTPs are flowed in an ordering that is not a continuous repeat of an ordering of the four different dNTPs. This alternate flow ordering may reduce the loss of phasic synchrony in the population of template polynucleotide strands that result from IE and/or CF errors.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.