Assay for determining a molecular risk assessment of a complex polymicrobial sample suspected to contain an EHEC

US9416425B2 · kind B2 · utility

Assignee

• Agence Nationale de Securite Sanitaire de l'Alimentation, de l'Environnement et due Travail · FR

Inventors

• Patrick Fach · Créteil, FR
• Marie Bugarel · Agen, FR
• Lothar Beutin · Berlin, DE

Key dates

• Filing date: Aug 11, 2010
• Grant date: Aug 16, 2016
• Expiry date: Oct 5, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/16
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a process to perform a molecular risk assessment (MRA) upon a sample suspected to contain an enterohemorrhagic Escherichia coli (EHEC), comprising the steps: contacting said sample or DNA isolated therefrom with a pair of primers derived from the following target genes stx1, stx2, eae and/or espk; wherein the process is characterized in that if amplification products are detected for each of the target genes in the first step, in a second step said sample or DNA isolated therefrom is contacted with a pair of primers derived from the following target genes nleB, nleH1-2, nleE, ent/espL2, eae subtypes γ, β, ε and θ and the target genes rfbE (0157), wbdl (0111), wzx (026); ihp1 (0145), wzx (0103); and detecting the presence or the absence of an amplification product for each of the target genes.