Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids

US9422593B2 · kind B2 · utility

Assignee

• QIAGEN GAITHRESBURG, INC · US

Inventors

• Thomas Rothmann · Langenfeld, DE
• Irina Nazarenko · Gaithersburg, US
• Dominic O'Neil · Hilden, DE
• Arvind Virmani · Germantown, US
• Brian Lowe · Greer, US
• Shiuli Agarwal · Somerville, US
• Holly Basham · Reisterstown, US

Key dates

• Filing date: May 18, 2011
• Grant date: Aug 23, 2016
• Expiry date: Jul 3, 2031

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods and materials for determining the presence of at least one nucleic acid in a sample are provided, said methods comprising (1) a purification step using sequence specific hybrid capture; (2) an amplification step; and (3) a detection step comprising contacting the target nucleic acid with a plurality of detectably labeled nucleic acid detection probes, wherein each (a) bears a different detectable label from the other detection probes, and/or (b) has a different melting temperature from probes bearing the same detectable label. Also disclosed are compositions and kits for use in such a method.