Cell culture of fetal liver in layered state in a partitioned micro-space
US9428721B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Feb 5, 2009 |
| Grant date | Aug 30, 2016 |
| Priority date | — |
| Expiry date | Dec 16, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5073
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided is a cell culture method whereby an in vivo function can be sustained over a long period of time and culture can be conducted using the minimum number of cells required. The cell culture method includes culturing undifferentiated cells in a layered state in a partitioned micro-space and obtains differentiated cells. When screening a pharmaceutical agent, undifferentiated cells capable of differentiating into liver cells, intestinal epithelial cells, nerve cells, myocardial cells and vascular endothelial cells are preferred. Particularly, in the prediction of pharmacokinetics or the like for humans, human cells are preferred.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.