Oscillating amplification reaction for nucleic acids
US9428781B2 · kind B2 · utility
21Cited by
31References
49Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 20, 2012 |
| Grant date | Aug 30, 2016 |
| Priority date | — |
| Expiry date | Apr 20, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6844
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
One embodiment of the present invention provides for a method for amplifying a template of nucleic acid target sequence contained in a sample. The method includes contacting the sample with an amplification reaction mixture containing a primer complementary to the template of nucleic acid target sequence. A temperature of the reaction is oscillated between an upper temperature and a lower temperature wherein the change in temperature is no greater than about 20° C. during a plurality of temperature cycles. The template of nucleic acid target sequence is amplified.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.