Polynucleotide and use thereof
US9428798B2 · kind B2 · utility
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14Claims
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Key dates
| Filing date | May 9, 2014 |
| Grant date | Aug 30, 2016 |
| Priority date | — |
| Expiry date | May 9, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6848
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A polynucleotide comprising a first region the 5′ end of which is complementary to a portion of a target nucleic acid, a cleavable second region, a third region having a stem-loop structure, and a fourth region complementary to the 3′ end of the first region, and use of the polynucleotide, as well as a composition comprising two such polynucleotides each of which hybridize different strands of a double-stranded target nucleic acid, and methods and kits using the same for amplifying targets.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.