Method for the production of multiple-unsaturated fatty acids in transgenic organisms

US9433228B2 · kind B2 · utility

Assignee

• BASF Plant Science Company GmbH · DE

Inventors

• Thorsten Zank · Mannheim, DE
• Jörg Bauer · Weingarten, DE
• Petra Cirpus · Mannheim, DE
• Amine Abbadi · Plettenberg, DE
• Ernst Heinz · Hamburg, DE
• Xiao Qiu · Beijing, CN
• Patricia Vrinten · Saskatoon, CA
• Petra Sperling · Hamburg, DE
• Frederic Domergue · Hamburg, DE
• Astrid Meyer
• Jelena Kirsch · Hamburg, DE

Key dates

• Filing date: Jul 16, 2004
• Grant date: Sep 6, 2016
• Expiry date: May 24, 2028

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02P20/52
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a method for the production of multiply-unsaturated fatty acids in an organism, into which nucleic acids have been introduced, which code for polypeptides with Δ-5 elongase activity. Said nucleic acid sequences, optionally with further nucleic acid sequences, coding for polypeptides for the biosynthesis of fatty acids and lipid metabolism, are advantageously expressed in the organism. Nucleic acid sequences coding for a Δ-6 desaturase, a Δ-5 desaturase, Δ-4 desaturase and/or Δ-6 elongase activity are particularly advantageous and, advantageously, said saturases and elongases are derived from Thalassiosira, Euglena or Ostreococcus. The invention further relates to a method for the production of oils and/or triacylglycerides with an increased content of long-chain, multiply-unsaturated fatty acids. A particular embodiment of the invention is a method for the production of unsaturated ω-3 fatty acids and a method for the production of triglycerides with an increased content of unsaturated fatty acids, in particular, of Δ-3 fatty acids with more than three double bonds. Also disclosed is the production of a transgenic organism, preferably a transgenic plant, or…