Gene from tidal flat metagenome and a novel protein displaying both phospholipase and lipase activities
US9441018B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 16, 2014 |
| Grant date | Sep 13, 2016 |
| Priority date | — |
| Expiry date | May 16, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y301/01032
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a novel gene derived from a tidal flat metagenome, and a novel protein obtained therefrom showing the coactivity of phospholipase and lipase. Specifically, the novel gene isolated from the metagenome library of tidal flat sediments and the protein having phospholipase and lipase activities encoded from the novel gene: are expressed in a water-soluble form to be mass-producible; enable ultra high-purity protein to be obtained through single step purification using an Ni-NTA column; show good activity in the pH range of 5˜10; maintain good low temperature activity and stability up to a temperature of 3° C. to 40° C.; and have high resistance against various organic solvents. Therefore, the novel gene and the protein can be usefully used for various industrial fields such as the purification and conversion of oil and fat, bio-medicine, and fine chemistry.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.