Methods and means for obtaining modified phenotypes

US9441239B2 · kind B2 · utility

Assignee

• COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION · AU

Inventors

• Peter Michael Waterhouse · Canberra, AU
• Ming-Bo Wang · Canberra, AU
• Michael Wayne Graham · Jindalee, AU

Key dates

• Filing date: Mar 1, 2006
• Grant date: Sep 13, 2016
• Expiry date: Mar 1, 2026

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K48/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods and means are provided for reducing the phenotypic expression of a nucleic acid of interest in eucaryotic cells, particularly in plant cells, by introducing chimeric genes encoding sense and antisense RNA molecules directed towards the target nucleic acid, which are capable of forming a double stranded RNA region by base-pairing between the regions with sense and antisense nucleotide sequence or by introducing the RNA molecules themselves. Preferably, the RNA molecules comprises simultaneously both sense and antisense nucleotide sequence.