Patent · US Active

Reduced artifact denaturing capillary electrophoresis of nucleic acids

US9442091B2 · kind B2 · utility

0Cited by
2References
20Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJan 30, 2012
Grant dateSep 13, 2016
Priority date—
Expiry dateNov 26, 2033

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/686
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Described are methods of reducing the incidence and/or magnitude of artifacts in denaturing nucleic acid capillary electrophoresis (CE). Methods and systems described serve to dismiss non-denatured DNA from the tip of the capillary after sample injection and prior to electrophoretic separation of loaded nucleic acids. Among the methods disclosed are the application of a brief reverse-polarity pulse to the capillary prior to separation but after removal of the capillary from the sample reservoir, and transiently heating the capillary to cause expansion of the separation matrix after removal of the capillary from the sample reservoir.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.