Method for creating perfusable microvessel systems
US9446169B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Aug 17, 2011 |
| Grant date | Sep 20, 2016 |
| Priority date | — |
| Expiry date | Feb 22, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/50
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for the creation of endothelial parent vessels from human vascular endothelial cells in vitro in a culture perfusion device (CPD) including a collagen chamber, inlet ports, a capillary tube, and an outlet port. A collagen solution is injected into the collagen chamber through a syringe needle until the chamber is filled with collagen. The CPD is perfused by filling the inlet ports and sequentially priming the inlet ports, and the outlet ports. A perfusable channel is created in the collagen chamber and a concentrated suspension of endothelial cells is injected into the inlet ports. The endothelial cells are injected into the at least one perfusable channel and incubated to attach to the walls of the perfusable channel. The cells are distributed within the CPD; and perfused to form a parent vessel having homogeneous monolayers of cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.