Method for accumulating protein in plant cells
US9447396B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 24, 2012 |
| Grant date | Sep 20, 2016 |
| Priority date | — |
| Expiry date | Sep 13, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/01
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
According to the present invention, a method for stably accumulating a target protein in plant cells or a plant body, and a transgenic plant in which protein has accumulated, are provided. The method of the present invention is a method for accumulating protein in plant cells, comprising accumulating a target protein or a protein deficient in an N-terminal region of the target protein in vacuoles of myrosin cells present in a multiple mutant, in which myrosin cells deficient in intravacuolar protein are also present in a plant body at locations other than around vascular bundles, by expressing a gene that encodes a target protein having an intracellular membrane system localization signal on the N-terminal and a vacuole localization signal on the C-terminal in the multiple mutant.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.