Methods and compositions for single cell expression profiling
US9447411B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Jan 13, 2014 |
| Grant date | Sep 20, 2016 |
| Priority date | — |
| Expiry date | Jan 13, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6886
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods of obtaining a single cell expression profile from a target mammalian cell are provided. Aspects of the methods include contacting a cellular sample which includes the target mammalian cell with a packaged viral barcoded trans-splicing library including a plurality of barcoded trans-splicing constructs under transduction conditions, where a barcoded trans-splicing construct includes a trans-splicing element linked to a barcode element. The methods further include generating expression data from the resultant transduced target mammalian cell to obtain the single cell expression data from the target mammalian cell. Also provided are compositions, e.g., libraries and components thereof, which find use in practicing the methods.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.