Detection of target nucleic acid sequences by cyclic exonucleolytic reactions
US9447457B2 · kind B2 · utility
10Cited by
1References
6Claims
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Key dates
| Filing date | Apr 9, 2010 |
| Grant date | Sep 20, 2016 |
| Priority date | — |
| Expiry date | Apr 9, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/682
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the detection of a target nucleic acid sequence by a cyclic exonucleolytic reaction. The present method enabling to generate signals by probe digestion with no help of primers and to amplify signals with no help of simultaneous target amplification reactions may enable to detect multiple target sequences without any problems accounted in the conventional real-time PCR methods such as false positive signals and difficulties in oligonucleotides (primer and probe) selection and reaction condition optimization.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.