Nanolipoprotein particles and related methods and systems for protein capture, solubilization, and/or purification
US9458191B2 · kind B2 · utility
6Cited by
7References
9Claims
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Key dates
| Filing date | Nov 7, 2014 |
| Grant date | Oct 4, 2016 |
| Priority date | — |
| Expiry date | Jan 23, 2035 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S977/799
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein are methods and systems for assembling, solubilizing and/or purifying a membrane associated protein in a nanolipoprotein particle, which comprise a temperature transition cycle performed in presence of a detergent, wherein during the temperature transition cycle the nanolipoprotein components are brought to a temperature above and below the gel to liquid crystalling transition temperature of the membrane forming lipid of the nanolipoprotein particle.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.