Modified DNA-binding proteins and uses thereof

US9458205B2 · kind B2 · utility

Assignee

• Sangamo Biosciences, Inc. · US

Inventors

• Philip D. Gregory · Richmond, US
• Jeffrey C. Miller · Pleasanton, US
• David Paschon · Oakland, US
• Edward J. Rebar · Richmond, US
• Siyuan Tan · Richmond, US
• Fyodor Urnov · Richmond, US
• Lei Zhang · Beijing, CN

Key dates

• Filing date: Nov 16, 2012
• Grant date: Oct 4, 2016
• Expiry date: Jul 17, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/63
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed herein are enhanced polypeptides, polynucleotides encoding these polypeptides, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences.