Secondary antibody detected epitope and preparation of same
US9458243B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 6, 2014 |
| Grant date | Oct 4, 2016 |
| Priority date | — |
| Expiry date | Dec 3, 2034 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2570/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of preparing an epitope for a secondary antibody detected protein tag, comprising: constructing a expressing vector, comprising a nucleotide sequence for encoding the secondary antibody detected protein tag comprising at least one epitope selected from at least one primary antibody which is detected by corresponding secondary antibody; expressing the vector in a host cell; and obtaining the secondary antibody detected protein tag which is expressed in the host cell. Further, a method for constructing a protein molecular weight marker ladder, comprising constructing a plurality of recombinant protein tags with different molecular weights, wherein each the recombinant protein tag comprises at least one epitope of primary antibody. Besides, a secondary antibody detected protein tag comprises at least two peptide sequences of epitopes selected from primary antibodies of at least two different species.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.