Optimized cellulase enzymes

US9458443B2 · kind B2 · utility

Assignee

• SUD-CHEMIE IP GMBH & CO. KG · DE

Inventors

• Ulrich Kettling
• Christoph Reisinger · Graz, AT
• Thomas Brück · Moosinning, DE
• Andre Koltermann · Icking, DE
• Jochen Gerlach · Munich, DE
• Isabel Unterstrasser · Rimsting, DE
• Lutz Röcher · Birkenfeld, DE
• Markus Rarbach · Cologne, DE
• Jörg CLAREN · Birkenfeld, DE
• Andreas Kohl · Birkenfeld, DE
• Jan Carsten Pieck · Wilnsdorf, DE
• Dominik Schlosser · Birkenfeld, DE

Key dates

• Filing date: Feb 11, 2011
• Grant date: Oct 4, 2016
• Expiry date: Nov 13, 2031

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02E50/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention discloses cellulase enzymes with optimized properties for processing of cellulose- and lignocellulose-containing substrates. In particular, cellobiohydrolase enzymes with preferred characteristics are disclosed. The present invention provides fusion, insertion, deletion and/or substitution variants of such enzymes. Enzyme variants have enhanced thermostability, proteolytic stability, specific activity and/or stability at extreme pH. Nucleic acid molecules encoding said enzymes, a composition comprising said enzymes, a method for preparation, and the use for cellulose processing and/or for the production of biofuels are disclosed.