Translational control system using RNA-protein interaction motif
US9458466B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 16, 2013 |
| Grant date | Oct 4, 2016 |
| Priority date | — |
| Expiry date | Jul 16, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/67
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A translational control method using an RNA-protein interaction motif is provided. The method comprises a step of introducing an mRNA having: a 5′UTR regulation structure comprising: (1) a cap structure at the 5′ terminus, (2) a spacer positioned on the 3′ side of the cap structure, and (3) one or more RNA motifs positioned on the 3′ side of the spacer, which comprises an RNA-protein interaction motif-derived nucleotide sequence or a variant thereof; and a nucleotide sequence encoding a target protein gene on the 3′ side of the 5′UTR regulation structure, into a cell in the presence of a protein specifically binding to the RNA motifs, wherein a translational level is decreased as the number of bases of the spacer decreases, and the translational level is decreased as the number of the RNA motifs increases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.