Method for the selective enrichment and labeling of phosphorproteins
US9464109B2 · kind B2 · utility
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11Claims
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Key dates
| Filing date | Sep 30, 2014 |
| Grant date | Oct 11, 2016 |
| Priority date | — |
| Expiry date | Sep 30, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K7/08
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for the introduction of a labeling structure such as fluorescent molecules or a Raman tag to a compound is described. Imidazole functionalized resins or polymers are used to selectively immobilize phosphocompounds without protecting the carboxylic groups is described. Relying on the pKa difference between amines and hydrazides and carrying out the reaction in a slightly acidic buffer, all of the amines are protected by protonation while the hydrazides react with the phosphate imidazolide to form a phosphoramidate bond.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.