Method for transcriptional amplification of nucleic acids combining steps of different temperatures
US9464308B2 · kind B2 · utility
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Key dates
| Filing date | Dec 14, 2012 |
| Grant date | Oct 11, 2016 |
| Priority date | — |
| Expiry date | Dec 14, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6865
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of transcriptional amplification includes: (a) obtaining a mixture by combining (i) a biological sample comprising nucleic acids, (ii) amplification primers, (iii) amplification reagents including enzymes required for amplification, and (iv) at least one polyol capable of stabilizing the enzymes required for amplification; (b) denaturing the nucleic acids by heating the mixture at a temperature above 41° C.; and (c) transcriptionally amplifying at least one target nucleic acid at a temperature above 41° C. when present in the mixture.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.