Chromatographic system and method of isolating compound in sample using chromatographic system

US9465016B2 · kind B2 · utility

Assignee

• AGILENT TECHNOLOGIES, INC. · US

Inventors

• Frank Wolf · Karlsruhe, DE
• Vlastimil Hruska · Waldbronn, DE
• Andreas Tei · Mannheim, DE
• Manuel Renz · Gettorf, DE

Key dates

• Filing date: Jan 14, 2014
• Grant date: Oct 11, 2016
• Expiry date: Dec 14, 2034

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2030/027
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method is provided for isolating a compound in a sample by chromatography. The method includes determining an analytical gradient of an analytical system; performing an analytical run using the analytical gradient; identifying a target compound in the sample and determining an analytical retention time of the target compound using chromatographic results of the analytical run; determining an elution point of the target compound using the analytical retention time, the analytical gradient and characteristics of the analytical system; determining automatically a focusing gradient of a preparative system, including a slope segment; and performing a preparative run using the focusing gradient to separate the target compound from the sample. The slope segment includes a slope determining a concentration of solvent in a solvent mixture, which linearly increases from a first offset below the elution point of the target compound to a second offset above the elution point of the target compound.