Cell culture process
US9475858B2 · kind B2 · utility
4Cited by
3References
38Claims
0Family size
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Key dates
| Filing date | Jul 6, 2012 |
| Grant date | Oct 25, 2016 |
| Priority date | — |
| Expiry date | Nov 1, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/14
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for producing recombinant polypeptides, including antibodies, having targeted levels of carboxyl terminal (C-terminal) lysine or arginine residues is presented. The method involves culturing cells expressing said polypeptides in cell culture medium having sufficient levels of lysine, arginine, and/or agents that change intracellular pH. Culturing the cells under such conditions does not affect cell growth, cell viability, or titer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.