Thermoresponsive substrate with microgels, method for its preparation and culture method for biological cells

US9476024B2 · kind B2 · utility

Assignee

• Fraunhofer-Gesellschaft zur förderung der angewandten Forschung e.V. · DE

Inventors

• Claus Duschl · Berlin, DE
• Andreas Lankenau · Rotkreuz, CH
• Stephan Schmidt · Falkensee, DE
• Thomas Hellweg · Bielefeld, DE
• Erik Wischerhoff · Potsdam, DE
• Andre Laschewsky · Potsdam, DE
• Jean-Francois Lutz · Kehl, DE

Key dates

• Filing date: Mar 21, 2011
• Grant date: Oct 25, 2016
• Expiry date: Dec 9, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2539/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to a culture substrate having thermoresponsive microgel particles that have a thermoresponsive polymer and modulator particles fixed to the carrier area of the substrate. The modulator can be a substance that has an adhesion capability with biological cells or a substance that causes cellular reactions that are inducible to binding to surface receptors of biological cells. Methods for preparation and use in culturing cells are given.