Patent · US Active

Method of producing isoprenoid compounds in yeast

US9476082B2 · kind B2 · utility

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22References
16Claims
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Key dates

Filing dateMay 20, 2011
Grant dateOct 25, 2016
Priority date—
Expiry dateMay 20, 2031

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY02P20/52
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Yeast strains capable of increased prenyl phosphate production are provided, enabling increased terpenoid molecule production. Heterologous yeast strains with high prenyl phosphate availability are prepared using one or both of two different strategies for increasing the availability of prenyl phosphates for terpenoid production. First, by co-expressing multiple mevalonate pathway gene analogs, a novel heterologous combination of genes results, some of which increases the inherent availability of prenyl phosphates in yeast. Second, by expressing the non-endogenous enzyme ATP citrate lysase (ACL), a buildup of high cytosolic concentration of acetyl-CoA is produced in the cytosol of S. cerevisiae.

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