Second harmonic imaging nanoprobes and techniques for use thereof
US9476830B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 21, 2007 |
| Grant date | Oct 25, 2016 |
| Priority date | — |
| Expiry date | Jan 30, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06V20/693
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Second harmonic nanoprobes for imaging biological samples and a method of using such probes to monitor the dynamics of biological process using a field resonance enhanced second harmonic (FRESH) technique are provided. The second harmonic generating (SHG) nanoprobes are comprised of various kinds of nanocrystals that do not possess an inversion symmetry and therefore are capable of generating second harmonic signals that can then be detected by conventional two-photon microscopy for in vivo imaging of biological processes and structures such as cell signaling, neuroimaging, protein conformation probing, DNA conformation probing, gene transcription, virus infection and replication in cells, protein dynamics, tumor imaging and cancer therapy evaluation and diagnosis as well as quantification in optical imaging.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.