Serum-free freezing medium used in adipose-derived stem cells and establishment of adipose-derived stem cell library
US9481865B2 · kind B2 · utility
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Key dates
| Filing date | Aug 6, 2012 |
| Grant date | Nov 1, 2016 |
| Priority date | — |
| Expiry date | Oct 11, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2501/999
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed is a serum-free freezing medium used in adipose-derived stem cells and a method for establishing an adipose-derived stem cell library. The serum-free freezing medium comprises a serum-free culture medium, dimethyl sulfoxide and a serum substitution component KSR; the defects of unstable freezing quality of the adipose-derived stem cells and influence of harmful factors in serum on the adipose-derived stem cells are solved, and the adipose-derived stem cells stored have the advantages of high survival percentage, well adherence growth and strong differentiation capacity.
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