Constitution of tool for analyzing biomolecular interaction and analysis method using same
US9481882B2 · kind B2 · utility
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Key dates
| Filing date | Mar 31, 2011 |
| Grant date | Nov 1, 2016 |
| Priority date | — |
| Expiry date | Feb 29, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/5308
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for analyzing biomolecular interactions, which method can be carried out without performing affinity selection using an immobilized bait, is provided. mRNA portions of assignment molecules that interacted with each other are linked together by annealing via a DNA linker for linking mRNA portions of assignment molecules together, the DNA linker comprising, at the 5′-end, an mRNA-complementary region complementary to a sequence at the 5′-end of each mRNA portion, and, at the 3′-end, a self-complementary region complementary between molecules of the DNA linker, the DNA linker being phosphorylated at the 5′-end.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.