Methods and devices for multiplexed microarray microfluidic analysis of biomolecules

US9481945B2 · kind B2 · utility

Assignee

• The Royal Institution for the Advancement of Learning/McGill University · CA

Inventors

• David Juncker · Zürich, CH
• Huiyan Li · Beijing, CN

Key dates

• Filing date: Aug 29, 2012
• Grant date: Nov 1, 2016
• Expiry date: Sep 27, 2032

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/6845
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Rapid and specific detection of biological cells and biomolecules is important to biological assays across diverse fields including genomics, proteomics, diagnoses, and pathological studies. Microarrays and microfluidics increasingly dominate such detection techniques due to the ability to perform significant numbers of tests with limited sample volumes. A snap chip assembly is provided for the transfer of a microarray of reagents within semi-spherical liquid droplets on a transfer chip to a target assay microarray on an assay chip following assembly of the two chips and physical contact of the droplets with the target array. Reagents in nanoliter quantities are spotted on both chips and selectively transferred as liquid droplets between transfer chip and assay chip within the contact areas. Using the snap chip structure the inventors performed immunoassays with colocalization of capture and detection antibodies with 10 targets and bead-in-gel droplet microarrays with 9 targets in the low pg/ml regime.