Method for regulating protein function in cells using synthetic small molecules

US9487787B2 · kind B2 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Thomas J. Wandless · Cambridge, US
• Laura A. Banaszynski · New York, US
• Mari Iwamoto · Stanford, US
• Lystranne Alysia Maynard · Silver Spring, US
• Ling Chen · Shanghai, CN

Key dates

• Filing date: Mar 22, 2012
• Grant date: Nov 8, 2016
• Expiry date: Mar 22, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/20
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods and compositions for the rapid and reversible destabilizing of specific proteins using cell-permeable, synthetic molecules are described. Stability-affecting proteins, e.g., derived from FKBP and DHFR proteins are fused to a protein of interest and the presence or absence of the ligand is used to modulate the stability of the fusion protein.