Patent · US Active

Method for lysing cells

US9493736B2 · kind B2 · utility

0Cited by

3References

13Claims

0Family size

Assignee

Merck Patent GmbH · DE

Inventors

Joerg Slaghuis · Darmstadt, DE
Peter Rossmanith · Baden, AT
Sabine Fuchs · Laa an der Thaya, AT
Patrick Julian Mester · Wien, AT
Martin Wagner · Madison, US

Key dates

Filing date	Mar 28, 2012
Grant date	Nov 15, 2016
Priority date	—
Expiry date	Mar 28, 2032

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6806
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention relates to a method for the lysis of cells, especially bacterial cells and the isolation of nucleic acids. The sample is treated with lysis solution that comprises at least one liquid that is not miscible with water and heated. Afterwards the mixture is cooled down and water is added so that after cooling a two phase system is generated. The nucleic acids can be found in the aqueous phase.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.