Materials and methods for profiling microRNAs
US9493825B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 16, 2012 |
| Grant date | Nov 15, 2016 |
| Priority date | — |
| Expiry date | Jul 6, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6876
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides materials and methods for detecting, quantifying, and/or high-throughput-profiling microRNAs. Advantageously, the present invention is more sensitive and specific than other currently-available miRNA qPCR assays. In addition, the present invention is convenient, easy-to-perform, and cost-effective. In one embodiment, the present invention provides a universal primer for reverse transcription of all miRNAs, a universal reverse primer for PCR amplification reaction, and universal probes. In another embodiment, the present invention provides assays that allow simultaneous detection and/or quantification of a plurality of target miRNAs using a single reverse transcription reaction.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.