Patent · US Active

Determination of in vivo DNA double-strand break localization and application thereof

US9493827B2 · kind B2 · utility

2Cited by

1References

15Claims

0Family size

Assignees

Deutsches Krebsforschungszentrum · DE
OSPEDALE SAN RAFFAELE S.R.L. · IT

Inventors

Christof Von Kalle · Glückstadt, DE
Manfred Schmidt · Krefeld, DE
Richard Gabriel · Schriesheim, DE
Ali Nowrouzi · Heidelberg, DE
Anne Arens · Schwetzingen, DE
Angelo Leone Lombardo · Milano, IT
Luigi Naldini · Torino, IT

Key dates

Filing date	Jan 13, 2011
Grant date	Nov 15, 2016
Priority date	—
Expiry date	Jan 13, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2740/15045
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention relates to a method for determining the in vivo localization of double-strand breaks in a host cell, comprising incubating a host cell suspected to comprise DNA double-strand breaks and a linear polynucleotide comprising a known sequence, detecting the in vivo insertion sites of said polynucleotide in the genome of said host cell, and assessing the in vivo localization of double-strand breaks. Further envisaged by the present invention is a method for obtaining an endonuclease with altered in vivo specificity. Finally, the present invention is directed to a kit for determining in vivo specificity of an endonuclease.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.