Patent · US Active

Single probe, multiple temperature, nucleic acid detection methods, kits, and compositions

US9493835B2 · kind B2 · utility

1Cited by

2References

4Claims

0Family size

Assignee

BRANDEIS UNIVERSITY · US

Inventors

Arthur Reis · Arlington, US
Lawrence J. Wangh · Waltham, US
Kenneth Pierce · Natick, US

Key dates

Filing date	Aug 11, 2010
Grant date	Nov 15, 2016
Priority date	—
Expiry date	Jan 20, 2033

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q2600/16
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Provided herein are methods, kits, and compositions related to nucleic acid detection assays that allow discrimination of multiple target sequences with a single probe. In particular, provided herein are methods kits, and compositions that include single-probe target sequence discrimination where different target amplicons may have identical probe hybridization sequences by employing multiple temperature end-point signal probe detection. Also provided herein are methods, kits, and compositions for distinguishing between two or more target amplicons using multiple-temperature end-point probe detection. In certain embodiments, asymmetric PCR amplification methods are employed (e.g., LATE-PCR amplification).

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.