DNA detection methods for site specific nuclease activity
US9493844B2 · kind B2 · utility
7Cited by
1References
20Claims
0Family size
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Key dates
| Filing date | Dec 13, 2013 |
| Grant date | Nov 15, 2016 |
| Priority date | — |
| Expiry date | Jan 25, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/156
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure provides methods for detecting and identifying plant events that contain precision targeted genomic loci, and plants and plant cells comprising such targeted genomic loci. The method can be deployed as a high throughput process utilized for screening the intactness or disruption of a targeted genomic loci and optionally for detecting a donor DNA polynucleotide insertion at the targeted genomic loci. The methods are readily applicable for the identification of plant events produced via a targeting method which results from the use of a site specific nuclease.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.