Methods for rapid multiplexed amplification of target nucleic acids

US9494519B2 · kind B2 · utility

Assignee

• NetBio, Inc. · US

Inventors

• Richard F. Selden · Wellesley, US
• Eugene Tan · Arlington, US
• Heung Chuan Lam · Newton, US
• Heidi Susanne Giese · Newburyport, US
• Gregory John Kellogg · Somerville, US
• John A. Wright · Billerica, US

Key dates

• Filing date: Mar 3, 2011
• Grant date: Nov 15, 2016
• Expiry date: Mar 3, 2031

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/2575
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A fast, multiplexed PCR system is described that can rapidly generate amplified nucleic acid products, for example, a full STR profile, from a target nucleic acid. Such systems include, for example, microfluidic biochips and a custom built thermal cycler, which are also described. The resulting STR profiles can satisfy forensic guidelines for signal strength, inter-loci peak height balance, heterozygous peak height ratio, incomplete non-template nucleotide addition, and stutter.