Cells screening method
US9494575B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Aug 1, 2008 |
| Grant date | Nov 15, 2016 |
| Priority date | — |
| Expiry date | Apr 12, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/715
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Provided are a method and means permitting the simultaneous measurement of the reactive properties of more than 10,000 of antigen-stimulated lymphocytes being held on a chip. A microwell array comprises multiple wells capturing single cells and a coating layer on a principal surface around the wells containing a substance capable of binding to a substance produced by the cells in the wells. A method for screening a target cell comprises: causing specimen cells and a cell culture broth to be contained in the wells of the microwell array; culturing the cells in a state permitting the diffusion of substances from the wells into the coating layer; feeding a label substance binding specifically to a substance produced by a target cell onto the coating layer; and detecting the substance produced by the target cell by the label substance binding around the well to specify the target cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.