Method for protein isolation in anoxic conditions
US9505802B2 · kind B2 · utility
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12References
4Claims
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Key dates
| Filing date | Jul 8, 2014 |
| Grant date | Nov 29, 2016 |
| Priority date | — |
| Expiry date | Aug 20, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/62
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for the isolation of proteins that comprise disulfide-bonds in their native conformation. Essentially, a method of the present makes the use of reducing agents such as β-mercaptoethanol or dithiothreitol in protein isolation methods obsolete. A method of the present invention is particularly suitable for the isolation of precursor proteins such as proinsulin from recombinant cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.