Compositions and methods for inducing myoblast differentiation and myotube formation

US9506033B2 · kind B2 · utility

Assignee

• University of Massachusetts · US

Inventors

• Michael P. Czech · Frankfurt am Main, DE
• Mengxi Wang · Nanhu, CN

Key dates

• Filing date: May 21, 2013
• Grant date: Nov 29, 2016
• Expiry date: May 21, 2033

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2800/10
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Provided herein are methods of inducing differentiation of a mammalian myoblast into a mammalian myocyte that include contacting a mammalian myoblast with an oligonucleotide that decreases Mitogen-activated protein kinase kinase kinase kinase 4 (Map4k4) mRNA expression in a mammalian myoblast or myocyte. Also provided are methods of inducing mammalian myoblasts or myocytes to form a myotube that include contacting two or more mammalian myoblasts or two or more mammalian myocytes with an oligonucleotide that decreases Map4k4 mRNA expression in a mammalian myoblast or myocyte. Also provided are methods of identifying a candidate agent useful for inducing muscle formation, and compositions containing an oligonucleotide that decreases Map4k4 mRNA expression in mammalian myoblast or myocyte and one or more additional muscle therapeutic agents and/or muscle-building neutraceuticals.