Processing of amplified DNA fragments for sequencing
US9506110B2 · kind B2 · utility
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8Claims
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Key dates
| Filing date | Dec 15, 2010 |
| Grant date | Nov 29, 2016 |
| Priority date | — |
| Expiry date | May 18, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6846
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A processing method to trim ends of DNA fragments, exposing the internal DNA part to give original DNA sequence information enabling application of next generation sequencing for DNA samples to be amplified by DOP-PCR or other primer dependent amplification methods. Specifically, nucleic acids are amplified using primers comprising a recognition site for a restriction enzyme, for example Bpml or Mmel. Primer sequences are removed by cleavage with the restriction enzyme.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.