Recombinant expression of multiprotein complexes using polygenes
US9512443B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 6, 2006 |
| Grant date | Dec 6, 2016 |
| Priority date | — |
| Expiry date | Dec 22, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2799/026
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a recombinant polynucleotide encoding a polygene coding for at least three polypeptides wherein at least one of the genes constituting the polygene is of non-viral origin, at least two of the polypeptides encoded by the genes constituting the polygene are each capable of at least transiently interacting with at least one other polypeptide encoded by a gene of said polygene, and the genes constituting the polygene are each connected to one mother by a sequence coding for at least one protease cleavage site. The present invention also relates to polyproteins encoded by the polygene. Further embodiments of the present invention are a vector containing the recombinant polypeptide, a host cell containing the recombinant polypeptide and/or the vector and a non-human transgenic animal transformed with the recombinant polypeptide and/or the vector. The present invention also relates to methods for the production of the polynucleotide and for the manufacture of multiprotein complexes. The embodiments of the present invention are particularly useful in gene therapy, drug candidate screening, vaccine production and crystallization of multiprotein complexes for…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.