Patent · US Active

Generating metal ion binding proteins

US9518131B2 · kind B2 · utility

0Cited by

2References

3Claims

0Family size

Assignee

Miltenyi Biotech, GmbH · DE

Inventors

Holger Thie · Biberach, DE
Kristina Reck · Kürten, DE
Volker Nolle · Kürten, DE

Key dates

Filing date	Apr 1, 2014
Grant date	Dec 13, 2016
Priority date	—
Expiry date	Apr 1, 2034

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2317/56
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention provides for a method for generating a metal ion binding protein, the method comprising a) integrating the amino acid sequence DDD into CDR1 of a light chain variable region of an antibody or fragment thereof; and b) combining the sequence of step a) with a heavy chain variable region of an antibody or fragment thereof; and c) isolating the protein. Also provided is the use of metal ion binding proteins generated by the method of the present invention for isolation and purification of proteins and for the reversible labeling of a target molecule. Also provided is a metal ion binding anti-CD8 protein.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.