Lentiviral-based vector and its use in directed evolution of genomic regions, genes and polynucleotides
US9518271B2 · kind B2 · utility
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Key dates
| Filing date | Nov 10, 2010 |
| Grant date | Dec 13, 2016 |
| Priority date | — |
| Expiry date | Feb 28, 2032 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/16062
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of directing evolution of a target polynucleotide of interest for obtaining variants of this target polynucleotide, a method to generate genetic variability by preparing a cell library, and a method to isolate or to screen variants of a polynucleotide or variants of a protein able to impact the phenotype of a cell or to confer a desired phenotype to target cells, and to identify theses polynucleotide variants or protein variants responsible for this phenotype are described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.