Method for decreasing pyruvate catabolism and increasing the accumulation of pyruvate in microbes
US9518275B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 26, 2014 |
| Grant date | Dec 13, 2016 |
| Priority date | — |
| Expiry date | Jan 12, 2035 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y203/01012
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method for decreasing pyruvate catabolism and increasing the accumulation of pyruvate in microbes. By overexpressing wild type dihydrolipoamide acetyltransferase or dihydrolipoamide acetyltransferase mutants which have mutations at conservative active sites, the present invention provide a method to decrease overall activity of pyruvate dehydrogenase complex and pyruvate catabolism, and thus increase the accumulation of extracellular pyruvate without killing the pyruvate-producing microbes. Overexpressing dihydrolipoamide acetyltransferase mutants is an effective way to increase pyruvate accumulation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.