Methods and compositions for identifying enterovirus

US9518303B2 · kind B2 · utility

Assignee

• The Board of Regents of the University of Texas System · US

Inventors

• Richard B. Pyles · Galveston, US
• Aaron Miller · Galveston, US

Key dates

• Filing date: Oct 25, 2012
• Grant date: Dec 13, 2016
• Expiry date: Oct 25, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/701
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Certain embodiments of the invention include, but are not limited to PCR primer pairs, sequencing primers, and/or associated thermocycling protocols targeting a region identified by the inventors within the 5′ untranslated region of enteroviruses for the purpose of identifying, subtyping, and/or classifying of virus in samples using nucleic acid sequencing. The sequencing procedures can use nucleic acid templates, such as cDNA or PCR amplicons, as a template for sequencing in medium to high throughput format that is cost effective and easily deployed to other clinical microbiology laboratories.