Method of detecting and/or quantifying an analyte in a biological sample
US9518986B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 2, 2012 |
| Grant date | Dec 13, 2016 |
| Priority date | — |
| Expiry date | Nov 2, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2610/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An aptamer-based SERS detection technique that directly monitors an aptamer-analyte capture event by generating spectroscopic information regarding the identity of the analyte that has been bound to the aptamer from a complex biological sample. A reproducible SERS spectrum is measured for an aptamer-analyte complex formed on a metal surface and this spectral information is used directly to identify the specific aptamer-analyte complex and optionally also to quantify the analyte in the sample, thus enabling discrimination between true and false positives in quantitative analyte assays on complex biological samples. In one embodiment the aptamer is attached directly to the metal surface and surrounded by a self-assembled monolayer (SAM) of amphiphilic molecules. In an alternative embodiment the metal surface is coated with a SAM and the aptamer is attached to the amphiphilic molecules of the SAM.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.