Method for preparing porous scaffold for tissue engineering, cell culture and cell delivery
US9522218B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Oct 10, 2008 |
| Grant date | Dec 20, 2016 |
| Priority date | — |
| Expiry date | Sep 6, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/74
- WIPO fieldMedical technology
- WIPO sectorInstruments
Abstract
The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprises the steps consisting of: a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide, an amount of a cross-linking agent and an amount of a porogen agent b) transforming the solution into a hydrogel by placing said solution at a temperature from about 4° C. to about 80° C. for a sufficient time to allow the cross-linking of said amount of polysaccharide and c) submerging said hydrogel into an aqueous solution d) washing the porous scaffold obtained at step c).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.